Use of nitrate, sulphate, and iron (III) as electron acceptors to improve the anaerobic degradation of linear alkylbenzene sulfonate: effects on removal potential and microbiota diversification.

Linear alkylbenzene sulfonate (LAS) is a synthetic anionic surfactant that is found in certain amounts in wastewaters and even in water bodies, despite its known biodegradability. This study aimed to assess the influence of nitrate, sulphate, and iron (III) on LAS anaerobic degradation and biomass microbial diversity. Batch reactors were inoculated with anaerobic biomass, nutrients, LAS (20 mg L-1), one of the three electron acceptors, and ethanol (40 mg L-1) as a co-substrate. The control treatments, with and without co-substrate, showed limited LAS biodegradation efficiencies of 10 ± 2% and 0%, respectively. However, when nitrate and iron (III) were present without co-substrate, biodegradation efficiencies of 53 ± 4% and 75 ± 3% were achieved, respectively, which were the highest levels observed. Clostridium spp. was prominent in all treatments, while Alkaliphilus spp. and Bacillus spp. thrived in the presence of iron, which had the most significant effect on LAS biodegradation. Those microorganisms were identified as crucial in affecting the LAS anaerobic degradation. The experiments revealed that the presence of electron acceptors fostered the development of a more specialised microbiota, especially those involved in the LAS biodegradation. A mutual interaction between the processes of degradation and adsorption was also shown.

Toxic effect of Croton rudolphianus leaf essential oil against Biomphalaria glabrata, Schistosoma mansoni cercariae and Artemia salina.

This research investigated the effect of the Croton rudolphianus leaf essential oil (EO) on Biomphalaria glabrata embryos (at different development stages) and adults, Schistosoma mansoni cercariae, and Artemia salina (non-target organism). It was possible to identify 31 compounds in the C. rudolphianus EO through GC-MS analysis. The major compounds from this oil were (E)-caryophyllene (17.33%), an unknown compound (16.87%), bicyclogermacrene (7.1%), δ-cadinene (6.62%) and germacrene D (5.38%). After incubation for 24 h, the EO of C. rudolphianus induced the occurrence of non-viable embryos (dead and malformed), with an LC50 value of 126.54, 133.51, 143.53 and 161.95 µg/mL and an LC90 value of 202.61, 216.48, 232.98 and 271.16 µg/mL to blastula, gastrula, trochophore and veliger embryonic stages, respectively. The EO was more effective against B. glabrata adults (LC50 and LC90 = 47.89 and 78.86 µg/mL, respectively), and S. mansoni cercariae (LC50 and LC90 = 14.81 and 22.15 after 120 mins of exposure, respectively) than against B. glabrata embryos. Concerning the micronucleus assay, the mean frequency of apoptosis, binucleation and micronucleus were 45.33 ± 3.51, 19.33 ± 1.53 and 0.67 ± 0.58 per 1000 cells at 25 µg/mL, which is the highest concentration tested. The oil killed A. salina with LC50 and LC90 values (68.33 and 111.5 µg/mL, respectively) higher than those determined for adult snails and S. mansoni cercariae. In conclusion, C. rudolphianus EO had a toxic effect against B. glabrata adults and embryos, and S. mansoni cercariae. Furthermore, this oil showed to be cytotoxic to hemocytes of B. glabrata. Concerning the non-target organism assay, C. rudolphianus EO was less toxic to A. salina then to adult snails and S. mansoni cercariae. Due to this, the EO from C. rudolphianus leaves is a potential alternative for schistosomiasis control.

1,3-Propanediol production from glycerol in polyurethane foam containing anaerobic reactors: performance and biomass cultivation and retention.

The use of batch and upflow anaerobic reactors filled with polyurethane foam for pure glycerol fermentation was evaluated. The best reactor operational conditions to obtain high yield and productivity of 1,3-propanediol (1,3-PDO) as the main product and the role of the polyurethane foam in the growth and retention of suspended and attached biomass in the reactors were investigated. In the experiment at 30 °C with a batch reactor (700 mL), biomass growth was mostly as immobilized attached cells, and the achieved 1,3-PDO yield was up to 0.58 mol mol-gly-1. In the experiment (30 °C) with an upflow anaerobic reactor (717 mL), glycerol loading rates (gly-LR) ranging from 6.94 to 15.47 g gly L-1 day-1 were applied during a 102-day period. During the operation, average 1,3-PDO yield was 0.47 mol mol-gly-1, reaching a maximum of 0.51 mol mol-gly-1 at gly-LR of 13.57 g gly L-1 day-1. High 1,3-PDO productivity (5.35 to 5.44 g L-1 day-1) was obtained when gly-LR was 13.57 to 15.47 g gly L-1 day-1. Comparing the close yield values in both batch and continuous reactors and based on microbial evaluation, it is concluded that most of the 1,3-PDO generated in the continuous reactor was due to the suspended biomass retained by the foam cubes. The Clostridium genus was the predominant 1,3-PDO producer. Good yields and productivities with packed reactors were attributed to polyurethane foam used for mixed culture growth and retention. Consequently, they are worth considering for 1,3-PDO production from pure glycerol.